Changes

              
    

          
          
        
        

            
f
1
{
f
1
{
            
2
  "author": "[{\"given_name\": \"Flurin\", \"name\": \"Leugger\", 
2
  "author": "[{\"given_name\": \"Flurin\", \"name\": \"Leugger\", 
            
3
\"email\": \"flurin.leugger@usys.ethz.ch\", \"data_credit\": 
3
\"email\": \"flurin.leugger@usys.ethz.ch\", \"data_credit\": 
            
4
[\"software\", \"curation\", \"collection\", \"validation\", 
4
[\"software\", \"curation\", \"collection\", \"validation\", 
            
5
\"publication\"], \"identifier\": \"\", \"affiliation\": \"WSL / 
5
\"publication\"], \"identifier\": \"\", \"affiliation\": \"WSL / 
            
6
ETH\"}, {\"given_name\": \"Martina\", \"name\": \"L\\u00fcthi\", 
6
ETH\"}, {\"given_name\": \"Martina\", \"name\": \"L\\u00fcthi\", 
            
7
\"email\": \"martina.luethi@usys.ethz.ch\", \"data_credit\": 
7
\"email\": \"martina.luethi@usys.ethz.ch\", \"data_credit\": 
            
8
[\"publication\", \"curation\", \"validation\", \"collection\", 
8
[\"publication\", \"curation\", \"validation\", \"collection\", 
            
9
\"supervision\"], \"identifier\": \"\", \"affiliation\": \"ETH / 
9
\"supervision\"], \"identifier\": \"\", \"affiliation\": \"ETH / 
            
10
WSL\"}, {\"given_name\": \"Michel\", \"name\": \"Schmidlin\", 
10
WSL\"}, {\"given_name\": \"Michel\", \"name\": \"Schmidlin\", 
            
11
\"email\": \"michelschmidlin@gmail.com\", \"data_credit\": 
11
\"email\": \"michelschmidlin@gmail.com\", \"data_credit\": 
            
12
[\"publication\", \"collection\", \"validation\"], \"identifier\": 
12
[\"publication\", \"collection\", \"validation\"], \"identifier\": 
            
13
\"\", \"affiliation\": \"ETH/WSL\"}, {\"given_name\": \"Zacharias\", 
13
\"\", \"affiliation\": \"ETH/WSL\"}, {\"given_name\": \"Zacharias\", 
            
14
\"name\": \"Kontarakis\", \"email\": 
14
\"name\": \"Kontarakis\", \"email\": 
            
15
\"zacharias.kontarakis@fgcz.ethz.ch\", \"data_credit\": 
15
\"zacharias.kontarakis@fgcz.ethz.ch\", \"data_credit\": 
            
16
[\"publication\", \"supervision\", \"validation\", \"curation\"], 
16
[\"publication\", \"supervision\", \"validation\", \"curation\"], 
            
17
\"identifier\": \"\", \"affiliation\": \"ETH\"}, {\"given_name\": 
17
\"identifier\": \"\", \"affiliation\": \"ETH\"}, {\"given_name\": 
            
18
\"Lo\\u00efc\", \"name\": \"Pellissier\", \"email\": 
18
\"Lo\\u00efc\", \"name\": \"Pellissier\", \"email\": 
            
19
\"loic.pellissier@usys.ethz.ch\", \"data_credit\": [\"publication\", 
19
\"loic.pellissier@usys.ethz.ch\", \"data_credit\": [\"publication\", 
            
20
\"supervision\", \"validation\", \"curation\"], \"identifier\": 
20
\"supervision\", \"validation\", \"curation\"], \"identifier\": 
            
21
\"0000-0002-2289-8259 \", \"affiliation\": \"Ecosystems and Landscape 
21
\"0000-0002-2289-8259 \", \"affiliation\": \"Ecosystems and Landscape 
            
22
Evolution, Dep. of Environmental Systems Science, ETH Zurich\"}]",
22
Evolution, Dep. of Environmental Systems Science, ETH Zurich\"}]",
            
23
  "author_email": null,
23
  "author_email": null,
            
24
  "creator_user_id": "29b58075-315f-4aa2-bfff-425ea9553e90",
24
  "creator_user_id": "29b58075-315f-4aa2-bfff-425ea9553e90",
            
25
  "date": 
25
  "date": 
            
26
:\"collected\",\"date\":\"2023-10-30\",\"end_date\":\"2023-11-10\"}]",
26
:\"collected\",\"date\":\"2023-10-30\",\"end_date\":\"2023-11-10\"}]",
            
27
  "doi": "10.16904/envidat.560",
27
  "doi": "10.16904/envidat.560",
            
28
  "funding": 
28
  "funding": 
            
29
\",\"institution_url\":\"https://data.snf.ch/grants/grant/203550\"}]",
29
\",\"institution_url\":\"https://data.snf.ch/grants/grant/203550\"}]",
            
30
  "groups": [],
30
  "groups": [],
            
31
  "id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
31
  "id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
            
32
  "isopen": true,
32
  "isopen": true,
            
33
  "license_id": "cc-by-sa",
33
  "license_id": "cc-by-sa",
            
34
  "license_title": "Creative Commons Attribution Share-Alike 
34
  "license_title": "Creative Commons Attribution Share-Alike 
            
35
(CC-BY-SA)",
35
(CC-BY-SA)",
            
36
  "license_url": "https://creativecommons.org/licenses/by-sa/4.0/",
36
  "license_url": "https://creativecommons.org/licenses/by-sa/4.0/",
            
37
  "maintainer": 
37
  "maintainer": 
            
38
ugger@usys.ethz.ch\",\"given_name\":\"Flurin\",\"name\":\"Leugger\"}",
38
ugger@usys.ethz.ch\",\"given_name\":\"Flurin\",\"name\":\"Leugger\"}",
            
39
  "maintainer_email": null,
39
  "maintainer_email": null,
            
40
  "metadata_created": "2024-11-27T16:58:50.243757",
40
  "metadata_created": "2024-11-27T16:58:50.243757",
            
n
41
  "metadata_modified": "2024-11-28T14:15:49.082307",
n
41
  "metadata_modified": "2024-11-29T06:46:18.872487",
            
42
  "name": 
42
  "name": 
            
43
d-edna-analysis-to-detect-the-threatened-and-elusive-african-manatee",
43
d-edna-analysis-to-detect-the-threatened-and-elusive-african-manatee",
            
44
  "notes": "# Data associated to the publication \"Rapid field-based 
44
  "notes": "# Data associated to the publication \"Rapid field-based 
            
45
detection of a threatened and elusive species with environmental DNA 
45
detection of a threatened and elusive species with environmental DNA 
            
46
and CRISPR-Dx\"\n\n<br> \n\n## Authors:\n\n<br>\n\nFlurin Leugger, 
46
and CRISPR-Dx\"\n\n<br> \n\n## Authors:\n\n<br>\n\nFlurin Leugger, 
            
47
Martina L\u00fcthi, Michel Schmidlin, Zacharias Kontarakis and 
47
Martina L\u00fcthi, Michel Schmidlin, Zacharias Kontarakis and 
            
48
Lo\u00efc Pellissier\n\n<br>\n\n## 
48
Lo\u00efc Pellissier\n\n<br>\n\n## 
            
49
n\nflurinleugger@gmail.com\nloic.pellissier@usys.ethz.ch\n\n<br>\n\n## 
49
n\nflurinleugger@gmail.com\nloic.pellissier@usys.ethz.ch\n\n<br>\n\n## 
            
50
Project description:\n\n<br>\n\nWe collected eDNA samples in the 
50
Project description:\n\n<br>\n\nWe collected eDNA samples in the 
            
51
Conkouati-Douli National Park in the Republic of Congo in 2023 in 
51
Conkouati-Douli National Park in the Republic of Congo in 2023 in 
            
52
collaboration with Beauval Nature and HELP Congo. We analyzed the 
52
collaboration with Beauval Nature and HELP Congo. We analyzed the 
            
53
samples on site in the national park using CRISPR-Dx assays to detect 
53
samples on site in the national park using CRISPR-Dx assays to detect 
            
54
the African manatee (*Trichechus senegalensis*). Additionally, we 
54
the African manatee (*Trichechus senegalensis*). Additionally, we 
            
55
transported the remaining samples (buffer) to Switzerland and used a 
55
transported the remaining samples (buffer) to Switzerland and used a 
            
56
high-sensitivity protocol with CRISPR-Dx assays and a qPCR assay to 
56
high-sensitivity protocol with CRISPR-Dx assays and a qPCR assay to 
            
57
verify the field-based detections. The qPCR of Hunter et al. (2018) 
57
verify the field-based detections. The qPCR of Hunter et al. (2018) 
            
58
detects the genus manatee (Trichechus). See publication for more 
58
detects the genus manatee (Trichechus). See publication for more 
            
59
details (doi will be added after publication).\n\n<br>\n\n## 
59
details (doi will be added after publication).\n\n<br>\n\n## 
            
60
Folders:\n\n<br>\n\n### lateral_flow_tests_images\\:\n\n<br>\n\nimages 
60
Folders:\n\n<br>\n\n### lateral_flow_tests_images\\:\n\n<br>\n\nimages 
            
61
of the lateral flow tests used to analyze the eDNA extracts in the 
61
of the lateral flow tests used to analyze the eDNA extracts in the 
            
62
national park. File names correspond to the site names. At each site, 
62
national park. File names correspond to the site names. At each site, 
            
63
we took two filter replicates. We ran 8 PCRs per filter/extract and 
63
we took two filter replicates. We ran 8 PCRs per filter/extract and 
            
64
pooled two PCRs together, resulting in 4 CRISPR-Dx assays per extract. 
64
pooled two PCRs together, resulting in 4 CRISPR-Dx assays per extract. 
            
65
The EVE number on top of the tests indicates the environmental extract 
65
The EVE number on top of the tests indicates the environmental extract 
            
66
number we use to track the extracts in our data base. It corresponds 
66
number we use to track the extracts in our data base. It corresponds 
            
67
to the extract_number_field_based in the metadata and data 
67
to the extract_number_field_based in the metadata and data 
            
68
folder.\n\n<br>\n\n### metadata\\:\n\n<br>\n\nFile containing overview 
68
folder.\n\n<br>\n\n### metadata\\:\n\n<br>\n\nFile containing overview 
            
69
of site names and extract number (field-based or lab-based 
69
of site names and extract number (field-based or lab-based 
            
70
extraction). The extract_number_field_based corresponds to the 
70
extraction). The extract_number_field_based corresponds to the 
            
71
identically called column in CRISPR_field_based_classifications.csv 
71
identically called column in CRISPR_field_based_classifications.csv 
            
72
file, as the extract_number_lab_based to the column in the file 
72
file, as the extract_number_lab_based to the column in the file 
            
73
CRISPR_lab_based_classifications.csv file and the 
73
CRISPR_lab_based_classifications.csv file and the 
            
74
qPCR_Cp_2nd_derivative.csv file. The file includes the GPS coordinates 
74
qPCR_Cp_2nd_derivative.csv file. The file includes the GPS coordinates 
            
75
of the sampling location (transect start point).\n\n<br>\n\n### 
75
of the sampling location (transect start point).\n\n<br>\n\n### 
            
76
data\\:\n\n<br>\n\nFolder containing the files with Cp values (qPCR) 
76
data\\:\n\n<br>\n\nFolder containing the files with Cp values (qPCR) 
            
77
and the CRISPR-Dx classifications. 
77
and the CRISPR-Dx classifications. 
            
78
CRISPR_field_based_classifications.csv file contains the 
78
CRISPR_field_based_classifications.csv file contains the 
            
79
classifications per pool per extract (0: no detection, 1: detection) 
79
classifications per pool per extract (0: no detection, 1: detection) 
            
80
for the African manatee (Trichechus 
80
for the African manatee (Trichechus 
            
81
senegalensis).\nCRISPR_lab_based_classifications.csv file contains the 
81
senegalensis).\nCRISPR_lab_based_classifications.csv file contains the 
            
82
classifications per pool per extract (0: no detection, 1: 
82
classifications per pool per extract (0: no detection, 1: 
            
83
detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of 
83
detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of 
            
84
the 8 replicates of each extract. \n",
84
the 8 replicates of each extract. \n",
            
85
  "num_resources": 4,
85
  "num_resources": 4,
            
86
  "num_tags": 9,
86
  "num_tags": 9,
            
87
  "organization": {
87
  "organization": {
            
88
    "approval_status": "approved",
88
    "approval_status": "approved",
            
89
    "created": "2018-12-01T11:59:01.791513",
89
    "created": "2018-12-01T11:59:01.791513",
            
90
    "description": "We are an international research team with diverse 
90
    "description": "We are an international research team with diverse 
            
91
scientific expertise and backgrounds, connected by the goal to 
91
scientific expertise and backgrounds, connected by the goal to 
            
92
understand biodiversity. We study the mechanisms that shape 
92
understand biodiversity. We study the mechanisms that shape 
            
93
biodiversity patterns across spatial and temporal scales - in both 
93
biodiversity patterns across spatial and temporal scales - in both 
            
94
terrestrial and aquatic ecosystems -\u200bsuch as mountain ranges and 
94
terrestrial and aquatic ecosystems -\u200bsuch as mountain ranges and 
            
95
tropical reefs. We use an interdisciplinary approach, whereby we 
95
tropical reefs. We use an interdisciplinary approach, whereby we 
            
96
bridge ecology, evolution, Earth history and global change.\r\nTo make 
96
bridge ecology, evolution, Earth history and global change.\r\nTo make 
            
97
these connections, we collect data through biological monitoring, 
97
these connections, we collect data through biological monitoring, 
            
98
environmental DNA methods, remote sensing, and field sampling, and use 
98
environmental DNA methods, remote sensing, and field sampling, and use 
            
99
these data to answer questions with statistical and 
99
these data to answer questions with statistical and 
            
100
process-\u200bbased models of biological diversity.\r\nOur group is 
100
process-\u200bbased models of biological diversity.\r\nOur group is 
            
101
affiliated with ETH and WSL, but is also associated with Agroscope, 
101
affiliated with ETH and WSL, but is also associated with Agroscope, 
            
102
EAWAG, the European Joint Research Centre and Vogelwarte.",
102
EAWAG, the European Joint Research Centre and Vogelwarte.",
            
103
    "id": "153392ee-d556-4b16-a330-dedb177e87f9",
103
    "id": "153392ee-d556-4b16-a330-dedb177e87f9",
            
104
    "image_url": 
104
    "image_url": 
            
105
le.ethz.ch/_jcr_content/orgbox/image.imageformat.logo.1254626489.png",
105
le.ethz.ch/_jcr_content/orgbox/image.imageformat.logo.1254626489.png",
            
106
    "is_organization": true,
106
    "is_organization": true,
            
107
    "name": "ele-group",
107
    "name": "ele-group",
            
108
    "state": "active",
108
    "state": "active",
            
109
    "title": "Ecosystems and Landscape Evolution",
109
    "title": "Ecosystems and Landscape Evolution",
            
110
    "type": "organization"
110
    "type": "organization"
            
111
  },
111
  },
            
112
  "owner_org": "153392ee-d556-4b16-a330-dedb177e87f9",
112
  "owner_org": "153392ee-d556-4b16-a330-dedb177e87f9",
            
113
  "private": false,
113
  "private": false,
            
114
  "publication": 
114
  "publication": 
            
115
"{\"publisher\":\"EnviDat\",\"publication_year\":\"2024\"}",
115
"{\"publisher\":\"EnviDat\",\"publication_year\":\"2024\"}",
            
116
  "publication_state": "published",
116
  "publication_state": "published",
            
117
  "relationships_as_object": [],
117
  "relationships_as_object": [],
            
118
  "relationships_as_subject": [],
118
  "relationships_as_subject": [],
            
119
  "resource_type": "dataset",
119
  "resource_type": "dataset",
            
120
  "resource_type_general": "dataset",
120
  "resource_type_general": "dataset",
            
121
  "resources": [
121
  "resources": [
            
122
    {
122
    {
            
123
      "cache_last_updated": null,
123
      "cache_last_updated": null,
            
124
      "cache_url": null,
124
      "cache_url": null,
            
125
      "created": "2024-11-28T13:25:32.642620",
125
      "created": "2024-11-28T13:25:32.642620",
            
126
      "description": "Description of project and folders and files",
126
      "description": "Description of project and folders and files",
            
127
      "doi": "",
127
      "doi": "",
            
128
      "format": "TXT",
128
      "format": "TXT",
            
129
      "hash": "",
129
      "hash": "",
            
130
      "id": "ad00caf8-bff2-4b98-be8b-122329cee39e",
130
      "id": "ad00caf8-bff2-4b98-be8b-122329cee39e",
            
131
      "last_modified": "2024-11-28T14:25:59.671000",
131
      "last_modified": "2024-11-28T14:25:59.671000",
            
132
      "metadata_modified": "2024-11-28T13:26:00.328522",
132
      "metadata_modified": "2024-11-28T13:26:00.328522",
            
133
      "mimetype": "text/plain",
133
      "mimetype": "text/plain",
            
134
      "mimetype_inner": null,
134
      "mimetype_inner": null,
            
135
      "name": "Readme.txt",
135
      "name": "Readme.txt",
            
136
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
136
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
            
137
      "position": 0,
137
      "position": 0,
            
138
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
138
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
            
139
      "resource_type": null,
139
      "resource_type": null,
            
140
      "restricted": 
140
      "restricted": 
            
141
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
141
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
            
142
      "size": 2387,
142
      "size": 2387,
            
143
      "state": "active",
143
      "state": "active",
            
144
      "url": 
144
      "url": 
            
145
56/resource/ad00caf8-bff2-4b98-be8b-122329cee39e/download/readme.txt",
145
56/resource/ad00caf8-bff2-4b98-be8b-122329cee39e/download/readme.txt",
            
146
      "url_type": "upload"
146
      "url_type": "upload"
            
147
    },
147
    },
            
148
    {
148
    {
            
149
      "cache_last_updated": null,
149
      "cache_last_updated": null,
            
150
      "cache_url": null,
150
      "cache_url": null,
            
151
      "created": "2024-11-28T13:26:13.642289",
151
      "created": "2024-11-28T13:26:13.642289",
            
152
      "description": "Folder containing the data files with Cp values 
152
      "description": "Folder containing the data files with Cp values 
            
153
(qPCR) and the CRISPR-Dx classifications. 
153
(qPCR) and the CRISPR-Dx classifications. 
            
154
CRISPR_field_based_classifications.csv file contains the 
154
CRISPR_field_based_classifications.csv file contains the 
            
155
classifications per pool per extract (0: no detection, 1: detection) 
155
classifications per pool per extract (0: no detection, 1: detection) 
            
156
for the African manatee (Trichechus 
156
for the African manatee (Trichechus 
            
157
senegalensis).\nCRISPR_lab_based_classifications.csv file contains the 
157
senegalensis).\nCRISPR_lab_based_classifications.csv file contains the 
            
158
classifications per pool per extract (0: no detection, 1: 
158
classifications per pool per extract (0: no detection, 1: 
            
159
detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of 
159
detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of 
            
160
the 8 replicates of each extract. ",
160
the 8 replicates of each extract. ",
            
161
      "doi": "",
161
      "doi": "",
            
162
      "format": "ZIP",
162
      "format": "ZIP",
            
163
      "hash": "",
163
      "hash": "",
            
164
      "id": "0b379b83-6480-4aca-b189-ff2435deb297",
164
      "id": "0b379b83-6480-4aca-b189-ff2435deb297",
            
165
      "last_modified": "2024-11-28T14:26:41.667000",
165
      "last_modified": "2024-11-28T14:26:41.667000",
            
166
      "metadata_modified": "2024-11-28T13:26:42.314004",
166
      "metadata_modified": "2024-11-28T13:26:42.314004",
            
167
      "mimetype": "application/zip",
167
      "mimetype": "application/zip",
            
168
      "mimetype_inner": null,
168
      "mimetype_inner": null,
            
169
      "name": "data.zip",
169
      "name": "data.zip",
            
170
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
170
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
            
171
      "position": 1,
171
      "position": 1,
            
172
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
172
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
            
173
      "resource_type": null,
173
      "resource_type": null,
            
174
      "restricted": 
174
      "restricted": 
            
175
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
175
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
            
176
      "size": 2126,
176
      "size": 2126,
            
177
      "state": "active",
177
      "state": "active",
            
178
      "url": 
178
      "url": 
            
179
ed56/resource/0b379b83-6480-4aca-b189-ff2435deb297/download/data.zip",
179
ed56/resource/0b379b83-6480-4aca-b189-ff2435deb297/download/data.zip",
            
180
      "url_type": "upload"
180
      "url_type": "upload"
            
181
    },
181
    },
            
182
    {
182
    {
            
183
      "cache_last_updated": null,
183
      "cache_last_updated": null,
            
184
      "cache_url": null,
184
      "cache_url": null,
            
185
      "created": "2024-11-28T13:27:04.698308",
185
      "created": "2024-11-28T13:27:04.698308",
            
186
      "description": "File containing overview of site names and 
186
      "description": "File containing overview of site names and 
            
187
extract number (field-based or lab-based extraction). The 
187
extract number (field-based or lab-based extraction). The 
            
188
extract_number_field_based corresponds to the identically called 
188
extract_number_field_based corresponds to the identically called 
            
189
column in CRISPR_field_based_classifications.csv file in the data 
189
column in CRISPR_field_based_classifications.csv file in the data 
            
190
folder, as the extract_number_lab_based to the column in the file 
190
folder, as the extract_number_lab_based to the column in the file 
            
191
CRISPR_lab_based_classifications.csv file and the 
191
CRISPR_lab_based_classifications.csv file and the 
            
192
qPCR_Cp_2nd_derivative.csv file (also in the data folder). The file 
192
qPCR_Cp_2nd_derivative.csv file (also in the data folder). The file 
            
193
includes the GPS coordinates of the sampling location (transect start 
193
includes the GPS coordinates of the sampling location (transect start 
            
194
point).",
194
point).",
            
195
      "doi": "",
195
      "doi": "",
            
196
      "format": "ZIP",
196
      "format": "ZIP",
            
197
      "hash": "",
197
      "hash": "",
            
198
      "id": "8501e9b9-e55f-49de-86ac-0754f15579dd",
198
      "id": "8501e9b9-e55f-49de-86ac-0754f15579dd",
            
199
      "last_modified": "2024-11-28T14:27:38.275000",
199
      "last_modified": "2024-11-28T14:27:38.275000",
            
200
      "metadata_modified": "2024-11-28T13:27:38.944594",
200
      "metadata_modified": "2024-11-28T13:27:38.944594",
            
201
      "mimetype": "application/zip",
201
      "mimetype": "application/zip",
            
202
      "mimetype_inner": null,
202
      "mimetype_inner": null,
            
203
      "name": "metadata.zip",
203
      "name": "metadata.zip",
            
204
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
204
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
            
205
      "position": 2,
205
      "position": 2,
            
206
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
206
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
            
207
      "resource_type": null,
207
      "resource_type": null,
            
208
      "restricted": 
208
      "restricted": 
            
209
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
209
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
            
210
      "size": 10066,
210
      "size": 10066,
            
211
      "state": "active",
211
      "state": "active",
            
212
      "url": 
212
      "url": 
            
213
/resource/8501e9b9-e55f-49de-86ac-0754f15579dd/download/metadata.zip",
213
/resource/8501e9b9-e55f-49de-86ac-0754f15579dd/download/metadata.zip",
            
214
      "url_type": "upload"
214
      "url_type": "upload"
            
215
    },
215
    },
            
216
    {
216
    {
            
217
      "cache_last_updated": null,
217
      "cache_last_updated": null,
            
218
      "cache_url": null,
218
      "cache_url": null,
            
219
      "created": "2024-11-28T13:27:45.362645",
219
      "created": "2024-11-28T13:27:45.362645",
            
220
      "description": "Images of the lateral flow tests used to analyze 
220
      "description": "Images of the lateral flow tests used to analyze 
            
221
the eDNA extracts in the national park. File names correspond to the 
221
the eDNA extracts in the national park. File names correspond to the 
            
222
site names. At each site, we took two filter replicates. We ran 8 PCRs 
222
site names. At each site, we took two filter replicates. We ran 8 PCRs 
            
223
per filter/extract and pooled two PCRs together, resulting in 4 
223
per filter/extract and pooled two PCRs together, resulting in 4 
            
224
CRISPR-Dx assays per extract. The EVE number on top of the tests 
224
CRISPR-Dx assays per extract. The EVE number on top of the tests 
            
225
indicates the environmental extract number we use to track the 
225
indicates the environmental extract number we use to track the 
            
226
extracts in our data base. It corresponds to the 
226
extracts in our data base. It corresponds to the 
            
n
227
extract_number_field_based in the metadata and data folder.",
n
227
extract_number_field_based in the metadata and data folder. The 
            
228
negative extraction control with the strongest \"band\" was placed 
            
229
right to the eDNA extract on each image (labelled hand written with NC 
            
230
x.y).",
            
228
      "doi": "",
231
      "doi": "",
            
229
      "format": "ZIP",
232
      "format": "ZIP",
            
230
      "hash": "",
233
      "hash": "",
            
231
      "id": "196f5458-7ced-49e3-a693-357ee2816f5a",
234
      "id": "196f5458-7ced-49e3-a693-357ee2816f5a",
            
t
232
      "last_modified": "2024-11-28T14:28:29.159000",
t
235
      "last_modified": "2024-11-29T07:46:18.177000",
            
233
      "metadata_modified": "2024-11-28T13:28:29.814170",
236
      "metadata_modified": "2024-11-29T06:46:18.885115",
            
234
      "mimetype": "application/zip",
237
      "mimetype": "application/zip",
            
235
      "mimetype_inner": null,
238
      "mimetype_inner": null,
            
236
      "name": "lateral_flow_tests_images.zip",
239
      "name": "lateral_flow_tests_images.zip",
            
237
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
240
      "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56",
            
238
      "position": 3,
241
      "position": 3,
            
239
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
242
      "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}",
            
240
      "resource_type": null,
243
      "resource_type": null,
            
241
      "restricted": 
244
      "restricted": 
            
242
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
245
{\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}",
            
243
      "size": 7430379,
246
      "size": 7430379,
            
244
      "state": "active",
247
      "state": "active",
            
245
      "url": 
248
      "url": 
            
246
8-7ced-49e3-a693-357ee2816f5a/download/lateral_flow_tests_images.zip",
249
8-7ced-49e3-a693-357ee2816f5a/download/lateral_flow_tests_images.zip",
            
247
      "url_type": "upload"
250
      "url_type": "upload"
            
248
    }
251
    }
            
249
  ],
252
  ],
            
250
  "spatial": 
253
  "spatial": 
            
251
na-analysis-to-detect-the-threatened-and-elusive-african-manatee\"}}",
254
na-analysis-to-detect-the-threatened-and-elusive-african-manatee\"}}",
            
252
  "state": "active",
255
  "state": "active",
            
253
  "subtitle": "",
256
  "subtitle": "",
            
254
  "tags": [
257
  "tags": [
            
255
    {
258
    {
            
256
      "display_name": "AFRICAN MANATEE",
259
      "display_name": "AFRICAN MANATEE",
            
257
      "id": "9493502c-2508-4a56-9848-eb579cced28a",
260
      "id": "9493502c-2508-4a56-9848-eb579cced28a",
            
258
      "name": "AFRICAN MANATEE",
261
      "name": "AFRICAN MANATEE",
            
259
      "state": "active",
262
      "state": "active",
            
260
      "vocabulary_id": null
263
      "vocabulary_id": null
            
261
    },
264
    },
            
262
    {
265
    {
            
263
      "display_name": "BIODIVERSITY MONITORING",
266
      "display_name": "BIODIVERSITY MONITORING",
            
264
      "id": "0ac3ee66-a65c-4d9a-ad05-b5b4193279da",
267
      "id": "0ac3ee66-a65c-4d9a-ad05-b5b4193279da",
            
265
      "name": "BIODIVERSITY MONITORING",
268
      "name": "BIODIVERSITY MONITORING",
            
266
      "state": "active",
269
      "state": "active",
            
267
      "vocabulary_id": null
270
      "vocabulary_id": null
            
268
    },
271
    },
            
269
    {
272
    {
            
270
      "display_name": "CRISPR-CAS",
273
      "display_name": "CRISPR-CAS",
            
271
      "id": "f94b68a2-d5c5-4dc7-b4d7-4b39fc27c86c",
274
      "id": "f94b68a2-d5c5-4dc7-b4d7-4b39fc27c86c",
            
272
      "name": "CRISPR-CAS",
275
      "name": "CRISPR-CAS",
            
273
      "state": "active",
276
      "state": "active",
            
274
      "vocabulary_id": null
277
      "vocabulary_id": null
            
275
    },
278
    },
            
276
    {
279
    {
            
277
      "display_name": "CRISPR-DX",
280
      "display_name": "CRISPR-DX",
            
278
      "id": "513b7a58-17f4-49d4-ae86-26713e2fcacd",
281
      "id": "513b7a58-17f4-49d4-ae86-26713e2fcacd",
            
279
      "name": "CRISPR-DX",
282
      "name": "CRISPR-DX",
            
280
      "state": "active",
283
      "state": "active",
            
281
      "vocabulary_id": null
284
      "vocabulary_id": null
            
282
    },
285
    },
            
283
    {
286
    {
            
284
      "display_name": "EDNA",
287
      "display_name": "EDNA",
            
285
      "id": "dab1a17e-9bf9-4e0a-9a60-c3ca8f8a9154",
288
      "id": "dab1a17e-9bf9-4e0a-9a60-c3ca8f8a9154",
            
286
      "name": "EDNA",
289
      "name": "EDNA",
            
287
      "state": "active",
290
      "state": "active",
            
288
      "vocabulary_id": null
291
      "vocabulary_id": null
            
289
    },
292
    },
            
290
    {
293
    {
            
291
      "display_name": "ENVIRONMENTAL DNA",
294
      "display_name": "ENVIRONMENTAL DNA",
            
292
      "id": "db7d5622-d1fc-4d24-91f7-73842c6743b6",
295
      "id": "db7d5622-d1fc-4d24-91f7-73842c6743b6",
            
293
      "name": "ENVIRONMENTAL DNA",
296
      "name": "ENVIRONMENTAL DNA",
            
294
      "state": "active",
297
      "state": "active",
            
295
      "vocabulary_id": null
298
      "vocabulary_id": null
            
296
    },
299
    },
            
297
    {
300
    {
            
298
      "display_name": "FIELD-BASED PROTOCOL",
301
      "display_name": "FIELD-BASED PROTOCOL",
            
299
      "id": "3fbda4ad-4504-4b3b-9ea5-d6fea74d8730",
302
      "id": "3fbda4ad-4504-4b3b-9ea5-d6fea74d8730",
            
300
      "name": "FIELD-BASED PROTOCOL",
303
      "name": "FIELD-BASED PROTOCOL",
            
301
      "state": "active",
304
      "state": "active",
            
302
      "vocabulary_id": null
305
      "vocabulary_id": null
            
303
    },
306
    },
            
304
    {
307
    {
            
305
      "display_name": "QPCR",
308
      "display_name": "QPCR",
            
306
      "id": "07ca9de3-732c-4f4b-9458-6bb5aaa124b2",
309
      "id": "07ca9de3-732c-4f4b-9458-6bb5aaa124b2",
            
307
      "name": "QPCR",
310
      "name": "QPCR",
            
308
      "state": "active",
311
      "state": "active",
            
309
      "vocabulary_id": null
312
      "vocabulary_id": null
            
310
    },
313
    },
            
311
    {
314
    {
            
312
      "display_name": "THREATENED SPECIES",
315
      "display_name": "THREATENED SPECIES",
            
313
      "id": "3429571a-7a40-4cf4-aa0d-ea6f69879ca7",
316
      "id": "3429571a-7a40-4cf4-aa0d-ea6f69879ca7",
            
314
      "name": "THREATENED SPECIES",
317
      "name": "THREATENED SPECIES",
            
315
      "state": "active",
318
      "state": "active",
            
316
      "vocabulary_id": null
319
      "vocabulary_id": null
            
317
    }
320
    }
            
318
  ],
321
  ],
            
319
  "title": "Field-based eDNA analysis to detect the threatened and 
322
  "title": "Field-based eDNA analysis to detect the threatened and 
            
320
elusive African manatee",
323
elusive African manatee",
            
321
  "type": "dataset",
324
  "type": "dataset",
            
322
  "url": null
325
  "url": null
            
323
}
326
}