Changes
On March 4, 2025 at 10:59:24 AM UTC,
-
Changed the version of Field-based eDNA analysis to detect the threatened and elusive African manatee to 1.0
f | 1 | { | f | 1 | { |
2 | "author": "[{\"given_name\": \"Flurin\", \"name\": \"Leugger\", | 2 | "author": "[{\"given_name\": \"Flurin\", \"name\": \"Leugger\", | ||
3 | \"email\": \"flurin.leugger@usys.ethz.ch\", \"data_credit\": | 3 | \"email\": \"flurin.leugger@usys.ethz.ch\", \"data_credit\": | ||
4 | [\"software\", \"curation\", \"collection\", \"validation\", | 4 | [\"software\", \"curation\", \"collection\", \"validation\", | ||
5 | \"publication\"], \"identifier\": \"\", \"affiliation\": \"WSL / | 5 | \"publication\"], \"identifier\": \"\", \"affiliation\": \"WSL / | ||
6 | ETH\"}, {\"given_name\": \"Martina\", \"name\": \"L\\u00fcthi\", | 6 | ETH\"}, {\"given_name\": \"Martina\", \"name\": \"L\\u00fcthi\", | ||
7 | \"email\": \"martina.luethi@usys.ethz.ch\", \"data_credit\": | 7 | \"email\": \"martina.luethi@usys.ethz.ch\", \"data_credit\": | ||
8 | [\"publication\", \"curation\", \"validation\", \"collection\", | 8 | [\"publication\", \"curation\", \"validation\", \"collection\", | ||
9 | \"supervision\"], \"identifier\": \"\", \"affiliation\": \"ETH / | 9 | \"supervision\"], \"identifier\": \"\", \"affiliation\": \"ETH / | ||
10 | WSL\"}, {\"given_name\": \"Michel\", \"name\": \"Schmidlin\", | 10 | WSL\"}, {\"given_name\": \"Michel\", \"name\": \"Schmidlin\", | ||
11 | \"email\": \"michelschmidlin@gmail.com\", \"data_credit\": | 11 | \"email\": \"michelschmidlin@gmail.com\", \"data_credit\": | ||
12 | [\"publication\", \"collection\", \"validation\"], \"identifier\": | 12 | [\"publication\", \"collection\", \"validation\"], \"identifier\": | ||
13 | \"\", \"affiliation\": \"ETH/WSL\"}, {\"given_name\": \"Zacharias\", | 13 | \"\", \"affiliation\": \"ETH/WSL\"}, {\"given_name\": \"Zacharias\", | ||
14 | \"name\": \"Kontarakis\", \"email\": | 14 | \"name\": \"Kontarakis\", \"email\": | ||
15 | \"zacharias.kontarakis@fgcz.ethz.ch\", \"data_credit\": | 15 | \"zacharias.kontarakis@fgcz.ethz.ch\", \"data_credit\": | ||
16 | [\"publication\", \"supervision\", \"validation\", \"curation\"], | 16 | [\"publication\", \"supervision\", \"validation\", \"curation\"], | ||
17 | \"identifier\": \"\", \"affiliation\": \"ETH\"}, {\"given_name\": | 17 | \"identifier\": \"\", \"affiliation\": \"ETH\"}, {\"given_name\": | ||
18 | \"Lo\\u00efc\", \"name\": \"Pellissier\", \"email\": | 18 | \"Lo\\u00efc\", \"name\": \"Pellissier\", \"email\": | ||
19 | \"loic.pellissier@usys.ethz.ch\", \"data_credit\": [\"publication\", | 19 | \"loic.pellissier@usys.ethz.ch\", \"data_credit\": [\"publication\", | ||
20 | \"supervision\", \"validation\", \"curation\"], \"identifier\": | 20 | \"supervision\", \"validation\", \"curation\"], \"identifier\": | ||
21 | \"0000-0002-2289-8259 \", \"affiliation\": \"Ecosystems and Landscape | 21 | \"0000-0002-2289-8259 \", \"affiliation\": \"Ecosystems and Landscape | ||
22 | Evolution, Dep. of Environmental Systems Science, ETH Zurich\"}]", | 22 | Evolution, Dep. of Environmental Systems Science, ETH Zurich\"}]", | ||
23 | "author_email": null, | 23 | "author_email": null, | ||
24 | "creator_user_id": "29b58075-315f-4aa2-bfff-425ea9553e90", | 24 | "creator_user_id": "29b58075-315f-4aa2-bfff-425ea9553e90", | ||
25 | "date": | 25 | "date": | ||
26 | :\"collected\",\"date\":\"2023-10-30\",\"end_date\":\"2023-11-10\"}]", | 26 | :\"collected\",\"date\":\"2023-10-30\",\"end_date\":\"2023-11-10\"}]", | ||
27 | "doi": "10.16904/envidat.560", | 27 | "doi": "10.16904/envidat.560", | ||
28 | "funding": | 28 | "funding": | ||
29 | \",\"institution_url\":\"https://data.snf.ch/grants/grant/203550\"}]", | 29 | \",\"institution_url\":\"https://data.snf.ch/grants/grant/203550\"}]", | ||
30 | "groups": [], | 30 | "groups": [], | ||
31 | "id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | 31 | "id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | ||
32 | "isopen": true, | 32 | "isopen": true, | ||
33 | "license_id": "cc-by-sa", | 33 | "license_id": "cc-by-sa", | ||
34 | "license_title": "Creative Commons Attribution Share-Alike | 34 | "license_title": "Creative Commons Attribution Share-Alike | ||
35 | (CC-BY-SA)", | 35 | (CC-BY-SA)", | ||
36 | "license_url": "https://creativecommons.org/licenses/by-sa/4.0/", | 36 | "license_url": "https://creativecommons.org/licenses/by-sa/4.0/", | ||
37 | "maintainer": | 37 | "maintainer": | ||
38 | ugger@usys.ethz.ch\",\"given_name\":\"Flurin\",\"name\":\"Leugger\"}", | 38 | ugger@usys.ethz.ch\",\"given_name\":\"Flurin\",\"name\":\"Leugger\"}", | ||
39 | "maintainer_email": null, | 39 | "maintainer_email": null, | ||
40 | "metadata_created": "2024-11-27T16:58:50.243757", | 40 | "metadata_created": "2024-11-27T16:58:50.243757", | ||
n | 41 | "metadata_modified": "2024-11-29T06:46:41.040275", | n | 41 | "metadata_modified": "2025-03-04T10:59:24.567965", |
42 | "name": | 42 | "name": | ||
43 | d-edna-analysis-to-detect-the-threatened-and-elusive-african-manatee", | 43 | d-edna-analysis-to-detect-the-threatened-and-elusive-african-manatee", | ||
44 | "notes": "# Data associated to the publication \"Rapid field-based | 44 | "notes": "# Data associated to the publication \"Rapid field-based | ||
45 | detection of a threatened and elusive species with environmental DNA | 45 | detection of a threatened and elusive species with environmental DNA | ||
46 | and CRISPR-Dx\"\n\n<br> \n\n## Authors:\n\n<br>\n\nFlurin Leugger, | 46 | and CRISPR-Dx\"\n\n<br> \n\n## Authors:\n\n<br>\n\nFlurin Leugger, | ||
47 | Martina L\u00fcthi, Michel Schmidlin, Zacharias Kontarakis and | 47 | Martina L\u00fcthi, Michel Schmidlin, Zacharias Kontarakis and | ||
48 | Lo\u00efc Pellissier\n\n<br>\n\n## | 48 | Lo\u00efc Pellissier\n\n<br>\n\n## | ||
49 | n\nflurinleugger@gmail.com\nloic.pellissier@usys.ethz.ch\n\n<br>\n\n## | 49 | n\nflurinleugger@gmail.com\nloic.pellissier@usys.ethz.ch\n\n<br>\n\n## | ||
50 | Project description:\n\n<br>\n\nWe collected eDNA samples in the | 50 | Project description:\n\n<br>\n\nWe collected eDNA samples in the | ||
51 | Conkouati-Douli National Park in the Republic of Congo in 2023 in | 51 | Conkouati-Douli National Park in the Republic of Congo in 2023 in | ||
52 | collaboration with Beauval Nature and HELP Congo. We analyzed the | 52 | collaboration with Beauval Nature and HELP Congo. We analyzed the | ||
53 | samples on site in the national park using CRISPR-Dx assays to detect | 53 | samples on site in the national park using CRISPR-Dx assays to detect | ||
54 | the African manatee (*Trichechus senegalensis*). Additionally, we | 54 | the African manatee (*Trichechus senegalensis*). Additionally, we | ||
55 | transported the remaining samples (buffer) to Switzerland and used a | 55 | transported the remaining samples (buffer) to Switzerland and used a | ||
56 | high-sensitivity protocol with CRISPR-Dx assays and a qPCR assay to | 56 | high-sensitivity protocol with CRISPR-Dx assays and a qPCR assay to | ||
57 | verify the field-based detections. The qPCR of Hunter et al. (2018) | 57 | verify the field-based detections. The qPCR of Hunter et al. (2018) | ||
58 | detects the genus manatee (Trichechus). See publication for more | 58 | detects the genus manatee (Trichechus). See publication for more | ||
59 | details (doi will be added after publication).\n\n<br>\n\n## | 59 | details (doi will be added after publication).\n\n<br>\n\n## | ||
60 | Folders:\n\n<br>\n\n### lateral_flow_tests_images\\:\n\n<br>\n\nImages | 60 | Folders:\n\n<br>\n\n### lateral_flow_tests_images\\:\n\n<br>\n\nImages | ||
61 | of the lateral flow tests used to analyze the eDNA extracts in the | 61 | of the lateral flow tests used to analyze the eDNA extracts in the | ||
62 | national park. File names correspond to the site names. At each site, | 62 | national park. File names correspond to the site names. At each site, | ||
63 | we took two filter replicates. We ran 8 PCRs per filter/extract and | 63 | we took two filter replicates. We ran 8 PCRs per filter/extract and | ||
64 | pooled two PCRs together, resulting in 4 CRISPR-Dx assays per extract. | 64 | pooled two PCRs together, resulting in 4 CRISPR-Dx assays per extract. | ||
65 | The EVE number on top of the tests indicates the environmental extract | 65 | The EVE number on top of the tests indicates the environmental extract | ||
66 | number we use to track the extracts in our data base. It corresponds | 66 | number we use to track the extracts in our data base. It corresponds | ||
67 | to the extract_number_field_based in the metadata and data folder. The | 67 | to the extract_number_field_based in the metadata and data folder. The | ||
68 | negative extraction control with the strongest \"band\" was placed | 68 | negative extraction control with the strongest \"band\" was placed | ||
69 | right to the eDNA extract on each image (labelled hand written with NC | 69 | right to the eDNA extract on each image (labelled hand written with NC | ||
70 | x.y).\n\n<br>\n\n### metadata\\:\n\n<br>\n\nFile containing overview | 70 | x.y).\n\n<br>\n\n### metadata\\:\n\n<br>\n\nFile containing overview | ||
71 | of site names and extract number (field-based or lab-based | 71 | of site names and extract number (field-based or lab-based | ||
72 | extraction). The extract_number_field_based corresponds to the | 72 | extraction). The extract_number_field_based corresponds to the | ||
73 | identically called column in CRISPR_field_based_classifications.csv | 73 | identically called column in CRISPR_field_based_classifications.csv | ||
74 | file, as the extract_number_lab_based to the column in the file | 74 | file, as the extract_number_lab_based to the column in the file | ||
75 | CRISPR_lab_based_classifications.csv file and the | 75 | CRISPR_lab_based_classifications.csv file and the | ||
76 | qPCR_Cp_2nd_derivative.csv file. The file includes the GPS coordinates | 76 | qPCR_Cp_2nd_derivative.csv file. The file includes the GPS coordinates | ||
77 | of the sampling location (transect start point).\n\n<br>\n\n### | 77 | of the sampling location (transect start point).\n\n<br>\n\n### | ||
78 | data\\:\n\n<br>\n\nFolder containing the files with Cp values (qPCR) | 78 | data\\:\n\n<br>\n\nFolder containing the files with Cp values (qPCR) | ||
79 | and the CRISPR-Dx classifications. | 79 | and the CRISPR-Dx classifications. | ||
80 | CRISPR_field_based_classifications.csv file contains the | 80 | CRISPR_field_based_classifications.csv file contains the | ||
81 | classifications per pool per extract (0: no detection, 1: detection) | 81 | classifications per pool per extract (0: no detection, 1: detection) | ||
82 | for the African manatee (Trichechus | 82 | for the African manatee (Trichechus | ||
83 | senegalensis).\nCRISPR_lab_based_classifications.csv file contains the | 83 | senegalensis).\nCRISPR_lab_based_classifications.csv file contains the | ||
84 | classifications per pool per extract (0: no detection, 1: | 84 | classifications per pool per extract (0: no detection, 1: | ||
85 | detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of | 85 | detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of | ||
86 | the 8 replicates of each extract. \n", | 86 | the 8 replicates of each extract. \n", | ||
87 | "num_resources": 4, | 87 | "num_resources": 4, | ||
88 | "num_tags": 9, | 88 | "num_tags": 9, | ||
89 | "organization": { | 89 | "organization": { | ||
90 | "approval_status": "approved", | 90 | "approval_status": "approved", | ||
91 | "created": "2018-12-01T11:59:01.791513", | 91 | "created": "2018-12-01T11:59:01.791513", | ||
92 | "description": "We are an international research team with diverse | 92 | "description": "We are an international research team with diverse | ||
93 | scientific expertise and backgrounds, connected by the goal to | 93 | scientific expertise and backgrounds, connected by the goal to | ||
94 | understand biodiversity. We study the mechanisms that shape | 94 | understand biodiversity. We study the mechanisms that shape | ||
95 | biodiversity patterns across spatial and temporal scales - in both | 95 | biodiversity patterns across spatial and temporal scales - in both | ||
96 | terrestrial and aquatic ecosystems -\u200bsuch as mountain ranges and | 96 | terrestrial and aquatic ecosystems -\u200bsuch as mountain ranges and | ||
97 | tropical reefs. We use an interdisciplinary approach, whereby we | 97 | tropical reefs. We use an interdisciplinary approach, whereby we | ||
98 | bridge ecology, evolution, Earth history and global change.\r\nTo make | 98 | bridge ecology, evolution, Earth history and global change.\r\nTo make | ||
99 | these connections, we collect data through biological monitoring, | 99 | these connections, we collect data through biological monitoring, | ||
100 | environmental DNA methods, remote sensing, and field sampling, and use | 100 | environmental DNA methods, remote sensing, and field sampling, and use | ||
101 | these data to answer questions with statistical and | 101 | these data to answer questions with statistical and | ||
102 | process-\u200bbased models of biological diversity.\r\nOur group is | 102 | process-\u200bbased models of biological diversity.\r\nOur group is | ||
103 | affiliated with ETH and WSL, but is also associated with Agroscope, | 103 | affiliated with ETH and WSL, but is also associated with Agroscope, | ||
104 | EAWAG, the European Joint Research Centre and Vogelwarte.", | 104 | EAWAG, the European Joint Research Centre and Vogelwarte.", | ||
105 | "id": "153392ee-d556-4b16-a330-dedb177e87f9", | 105 | "id": "153392ee-d556-4b16-a330-dedb177e87f9", | ||
106 | "image_url": | 106 | "image_url": | ||
107 | le.ethz.ch/_jcr_content/orgbox/image.imageformat.logo.1254626489.png", | 107 | le.ethz.ch/_jcr_content/orgbox/image.imageformat.logo.1254626489.png", | ||
108 | "is_organization": true, | 108 | "is_organization": true, | ||
109 | "name": "ele-group", | 109 | "name": "ele-group", | ||
110 | "state": "active", | 110 | "state": "active", | ||
111 | "title": "Ecosystems and Landscape Evolution", | 111 | "title": "Ecosystems and Landscape Evolution", | ||
112 | "type": "organization" | 112 | "type": "organization" | ||
113 | }, | 113 | }, | ||
114 | "owner_org": "153392ee-d556-4b16-a330-dedb177e87f9", | 114 | "owner_org": "153392ee-d556-4b16-a330-dedb177e87f9", | ||
115 | "private": false, | 115 | "private": false, | ||
116 | "publication": | 116 | "publication": | ||
117 | "{\"publisher\":\"EnviDat\",\"publication_year\":\"2024\"}", | 117 | "{\"publisher\":\"EnviDat\",\"publication_year\":\"2024\"}", | ||
118 | "publication_state": "published", | 118 | "publication_state": "published", | ||
119 | "relationships_as_object": [], | 119 | "relationships_as_object": [], | ||
120 | "relationships_as_subject": [], | 120 | "relationships_as_subject": [], | ||
121 | "resource_type": "dataset", | 121 | "resource_type": "dataset", | ||
122 | "resource_type_general": "dataset", | 122 | "resource_type_general": "dataset", | ||
123 | "resources": [ | 123 | "resources": [ | ||
124 | { | 124 | { | ||
125 | "cache_last_updated": null, | 125 | "cache_last_updated": null, | ||
126 | "cache_url": null, | 126 | "cache_url": null, | ||
127 | "created": "2024-11-28T13:25:32.642620", | 127 | "created": "2024-11-28T13:25:32.642620", | ||
128 | "description": "Description of project and folders and files", | 128 | "description": "Description of project and folders and files", | ||
129 | "doi": "", | 129 | "doi": "", | ||
130 | "format": "TXT", | 130 | "format": "TXT", | ||
131 | "hash": "", | 131 | "hash": "", | ||
132 | "id": "ad00caf8-bff2-4b98-be8b-122329cee39e", | 132 | "id": "ad00caf8-bff2-4b98-be8b-122329cee39e", | ||
133 | "last_modified": "2024-11-28T14:25:59.671000", | 133 | "last_modified": "2024-11-28T14:25:59.671000", | ||
134 | "metadata_modified": "2024-11-28T13:26:00.328522", | 134 | "metadata_modified": "2024-11-28T13:26:00.328522", | ||
135 | "mimetype": "text/plain", | 135 | "mimetype": "text/plain", | ||
136 | "mimetype_inner": null, | 136 | "mimetype_inner": null, | ||
137 | "name": "Readme.txt", | 137 | "name": "Readme.txt", | ||
138 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | 138 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | ||
139 | "position": 0, | 139 | "position": 0, | ||
140 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | 140 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | ||
141 | "resource_type": null, | 141 | "resource_type": null, | ||
142 | "restricted": | 142 | "restricted": | ||
143 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | 143 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | ||
144 | "size": 2387, | 144 | "size": 2387, | ||
145 | "state": "active", | 145 | "state": "active", | ||
146 | "url": | 146 | "url": | ||
147 | 56/resource/ad00caf8-bff2-4b98-be8b-122329cee39e/download/readme.txt", | 147 | 56/resource/ad00caf8-bff2-4b98-be8b-122329cee39e/download/readme.txt", | ||
148 | "url_type": "upload" | 148 | "url_type": "upload" | ||
149 | }, | 149 | }, | ||
150 | { | 150 | { | ||
151 | "cache_last_updated": null, | 151 | "cache_last_updated": null, | ||
152 | "cache_url": null, | 152 | "cache_url": null, | ||
153 | "created": "2024-11-28T13:26:13.642289", | 153 | "created": "2024-11-28T13:26:13.642289", | ||
154 | "description": "Folder containing the data files with Cp values | 154 | "description": "Folder containing the data files with Cp values | ||
155 | (qPCR) and the CRISPR-Dx classifications. | 155 | (qPCR) and the CRISPR-Dx classifications. | ||
156 | CRISPR_field_based_classifications.csv file contains the | 156 | CRISPR_field_based_classifications.csv file contains the | ||
157 | classifications per pool per extract (0: no detection, 1: detection) | 157 | classifications per pool per extract (0: no detection, 1: detection) | ||
158 | for the African manatee (Trichechus | 158 | for the African manatee (Trichechus | ||
159 | senegalensis).\nCRISPR_lab_based_classifications.csv file contains the | 159 | senegalensis).\nCRISPR_lab_based_classifications.csv file contains the | ||
160 | classifications per pool per extract (0: no detection, 1: | 160 | classifications per pool per extract (0: no detection, 1: | ||
161 | detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of | 161 | detection).\nqPCR_Cp_2nd_derivative.csv file contains the Cp value of | ||
162 | the 8 replicates of each extract. ", | 162 | the 8 replicates of each extract. ", | ||
163 | "doi": "", | 163 | "doi": "", | ||
164 | "format": "ZIP", | 164 | "format": "ZIP", | ||
165 | "hash": "", | 165 | "hash": "", | ||
166 | "id": "0b379b83-6480-4aca-b189-ff2435deb297", | 166 | "id": "0b379b83-6480-4aca-b189-ff2435deb297", | ||
167 | "last_modified": "2024-11-28T14:26:41.667000", | 167 | "last_modified": "2024-11-28T14:26:41.667000", | ||
168 | "metadata_modified": "2024-11-28T13:26:42.314004", | 168 | "metadata_modified": "2024-11-28T13:26:42.314004", | ||
169 | "mimetype": "application/zip", | 169 | "mimetype": "application/zip", | ||
170 | "mimetype_inner": null, | 170 | "mimetype_inner": null, | ||
171 | "name": "data.zip", | 171 | "name": "data.zip", | ||
172 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | 172 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | ||
173 | "position": 1, | 173 | "position": 1, | ||
174 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | 174 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | ||
175 | "resource_type": null, | 175 | "resource_type": null, | ||
176 | "restricted": | 176 | "restricted": | ||
177 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | 177 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | ||
178 | "size": 2126, | 178 | "size": 2126, | ||
179 | "state": "active", | 179 | "state": "active", | ||
180 | "url": | 180 | "url": | ||
181 | ed56/resource/0b379b83-6480-4aca-b189-ff2435deb297/download/data.zip", | 181 | ed56/resource/0b379b83-6480-4aca-b189-ff2435deb297/download/data.zip", | ||
182 | "url_type": "upload" | 182 | "url_type": "upload" | ||
183 | }, | 183 | }, | ||
184 | { | 184 | { | ||
185 | "cache_last_updated": null, | 185 | "cache_last_updated": null, | ||
186 | "cache_url": null, | 186 | "cache_url": null, | ||
187 | "created": "2024-11-28T13:27:04.698308", | 187 | "created": "2024-11-28T13:27:04.698308", | ||
188 | "description": "File containing overview of site names and | 188 | "description": "File containing overview of site names and | ||
189 | extract number (field-based or lab-based extraction). The | 189 | extract number (field-based or lab-based extraction). The | ||
190 | extract_number_field_based corresponds to the identically called | 190 | extract_number_field_based corresponds to the identically called | ||
191 | column in CRISPR_field_based_classifications.csv file in the data | 191 | column in CRISPR_field_based_classifications.csv file in the data | ||
192 | folder, as the extract_number_lab_based to the column in the file | 192 | folder, as the extract_number_lab_based to the column in the file | ||
193 | CRISPR_lab_based_classifications.csv file and the | 193 | CRISPR_lab_based_classifications.csv file and the | ||
194 | qPCR_Cp_2nd_derivative.csv file (also in the data folder). The file | 194 | qPCR_Cp_2nd_derivative.csv file (also in the data folder). The file | ||
195 | includes the GPS coordinates of the sampling location (transect start | 195 | includes the GPS coordinates of the sampling location (transect start | ||
196 | point).", | 196 | point).", | ||
197 | "doi": "", | 197 | "doi": "", | ||
198 | "format": "ZIP", | 198 | "format": "ZIP", | ||
199 | "hash": "", | 199 | "hash": "", | ||
200 | "id": "8501e9b9-e55f-49de-86ac-0754f15579dd", | 200 | "id": "8501e9b9-e55f-49de-86ac-0754f15579dd", | ||
201 | "last_modified": "2024-11-28T14:27:38.275000", | 201 | "last_modified": "2024-11-28T14:27:38.275000", | ||
202 | "metadata_modified": "2024-11-28T13:27:38.944594", | 202 | "metadata_modified": "2024-11-28T13:27:38.944594", | ||
203 | "mimetype": "application/zip", | 203 | "mimetype": "application/zip", | ||
204 | "mimetype_inner": null, | 204 | "mimetype_inner": null, | ||
205 | "name": "metadata.zip", | 205 | "name": "metadata.zip", | ||
206 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | 206 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | ||
207 | "position": 2, | 207 | "position": 2, | ||
208 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | 208 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | ||
209 | "resource_type": null, | 209 | "resource_type": null, | ||
210 | "restricted": | 210 | "restricted": | ||
211 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | 211 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | ||
212 | "size": 10066, | 212 | "size": 10066, | ||
213 | "state": "active", | 213 | "state": "active", | ||
214 | "url": | 214 | "url": | ||
215 | /resource/8501e9b9-e55f-49de-86ac-0754f15579dd/download/metadata.zip", | 215 | /resource/8501e9b9-e55f-49de-86ac-0754f15579dd/download/metadata.zip", | ||
216 | "url_type": "upload" | 216 | "url_type": "upload" | ||
217 | }, | 217 | }, | ||
218 | { | 218 | { | ||
219 | "cache_last_updated": null, | 219 | "cache_last_updated": null, | ||
220 | "cache_url": null, | 220 | "cache_url": null, | ||
221 | "created": "2024-11-28T13:27:45.362645", | 221 | "created": "2024-11-28T13:27:45.362645", | ||
222 | "description": "Images of the lateral flow tests used to analyze | 222 | "description": "Images of the lateral flow tests used to analyze | ||
223 | the eDNA extracts in the national park. File names correspond to the | 223 | the eDNA extracts in the national park. File names correspond to the | ||
224 | site names. At each site, we took two filter replicates. We ran 8 PCRs | 224 | site names. At each site, we took two filter replicates. We ran 8 PCRs | ||
225 | per filter/extract and pooled two PCRs together, resulting in 4 | 225 | per filter/extract and pooled two PCRs together, resulting in 4 | ||
226 | CRISPR-Dx assays per extract. The EVE number on top of the tests | 226 | CRISPR-Dx assays per extract. The EVE number on top of the tests | ||
227 | indicates the environmental extract number we use to track the | 227 | indicates the environmental extract number we use to track the | ||
228 | extracts in our data base. It corresponds to the | 228 | extracts in our data base. It corresponds to the | ||
229 | extract_number_field_based in the metadata and data folder. The | 229 | extract_number_field_based in the metadata and data folder. The | ||
230 | negative extraction control with the strongest \"band\" was placed | 230 | negative extraction control with the strongest \"band\" was placed | ||
231 | right to the eDNA extract on each image (labelled hand written with NC | 231 | right to the eDNA extract on each image (labelled hand written with NC | ||
232 | x.y).", | 232 | x.y).", | ||
233 | "doi": "", | 233 | "doi": "", | ||
234 | "format": "ZIP", | 234 | "format": "ZIP", | ||
235 | "hash": "", | 235 | "hash": "", | ||
236 | "id": "196f5458-7ced-49e3-a693-357ee2816f5a", | 236 | "id": "196f5458-7ced-49e3-a693-357ee2816f5a", | ||
237 | "last_modified": "2024-11-29T07:46:18.177000", | 237 | "last_modified": "2024-11-29T07:46:18.177000", | ||
238 | "metadata_modified": "2024-11-29T06:46:18.885115", | 238 | "metadata_modified": "2024-11-29T06:46:18.885115", | ||
239 | "mimetype": "application/zip", | 239 | "mimetype": "application/zip", | ||
240 | "mimetype_inner": null, | 240 | "mimetype_inner": null, | ||
241 | "name": "lateral_flow_tests_images.zip", | 241 | "name": "lateral_flow_tests_images.zip", | ||
242 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | 242 | "package_id": "4a99830f-a254-4179-a490-d7ca76c5ed56", | ||
243 | "position": 3, | 243 | "position": 3, | ||
244 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | 244 | "resource_size": "{\"size_value\":\"\",\"size_units\":\"\"}", | ||
245 | "resource_type": null, | 245 | "resource_type": null, | ||
246 | "restricted": | 246 | "restricted": | ||
247 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | 247 | {\"level\":\"public\",\"allowed_users\":\"\",\"shared_secret\":\"\"}", | ||
248 | "size": 7430379, | 248 | "size": 7430379, | ||
249 | "state": "active", | 249 | "state": "active", | ||
250 | "url": | 250 | "url": | ||
251 | 8-7ced-49e3-a693-357ee2816f5a/download/lateral_flow_tests_images.zip", | 251 | 8-7ced-49e3-a693-357ee2816f5a/download/lateral_flow_tests_images.zip", | ||
252 | "url_type": "upload" | 252 | "url_type": "upload" | ||
253 | } | 253 | } | ||
254 | ], | 254 | ], | ||
255 | "spatial": | 255 | "spatial": | ||
256 | na-analysis-to-detect-the-threatened-and-elusive-african-manatee\"}}", | 256 | na-analysis-to-detect-the-threatened-and-elusive-african-manatee\"}}", | ||
257 | "state": "active", | 257 | "state": "active", | ||
258 | "subtitle": "", | 258 | "subtitle": "", | ||
259 | "tags": [ | 259 | "tags": [ | ||
260 | { | 260 | { | ||
261 | "display_name": "AFRICAN MANATEE", | 261 | "display_name": "AFRICAN MANATEE", | ||
262 | "id": "9493502c-2508-4a56-9848-eb579cced28a", | 262 | "id": "9493502c-2508-4a56-9848-eb579cced28a", | ||
263 | "name": "AFRICAN MANATEE", | 263 | "name": "AFRICAN MANATEE", | ||
264 | "state": "active", | 264 | "state": "active", | ||
265 | "vocabulary_id": null | 265 | "vocabulary_id": null | ||
266 | }, | 266 | }, | ||
267 | { | 267 | { | ||
268 | "display_name": "BIODIVERSITY MONITORING", | 268 | "display_name": "BIODIVERSITY MONITORING", | ||
269 | "id": "0ac3ee66-a65c-4d9a-ad05-b5b4193279da", | 269 | "id": "0ac3ee66-a65c-4d9a-ad05-b5b4193279da", | ||
270 | "name": "BIODIVERSITY MONITORING", | 270 | "name": "BIODIVERSITY MONITORING", | ||
271 | "state": "active", | 271 | "state": "active", | ||
272 | "vocabulary_id": null | 272 | "vocabulary_id": null | ||
273 | }, | 273 | }, | ||
274 | { | 274 | { | ||
275 | "display_name": "CRISPR-CAS", | 275 | "display_name": "CRISPR-CAS", | ||
276 | "id": "f94b68a2-d5c5-4dc7-b4d7-4b39fc27c86c", | 276 | "id": "f94b68a2-d5c5-4dc7-b4d7-4b39fc27c86c", | ||
277 | "name": "CRISPR-CAS", | 277 | "name": "CRISPR-CAS", | ||
278 | "state": "active", | 278 | "state": "active", | ||
279 | "vocabulary_id": null | 279 | "vocabulary_id": null | ||
280 | }, | 280 | }, | ||
281 | { | 281 | { | ||
282 | "display_name": "CRISPR-DX", | 282 | "display_name": "CRISPR-DX", | ||
283 | "id": "513b7a58-17f4-49d4-ae86-26713e2fcacd", | 283 | "id": "513b7a58-17f4-49d4-ae86-26713e2fcacd", | ||
284 | "name": "CRISPR-DX", | 284 | "name": "CRISPR-DX", | ||
285 | "state": "active", | 285 | "state": "active", | ||
286 | "vocabulary_id": null | 286 | "vocabulary_id": null | ||
287 | }, | 287 | }, | ||
288 | { | 288 | { | ||
289 | "display_name": "EDNA", | 289 | "display_name": "EDNA", | ||
290 | "id": "dab1a17e-9bf9-4e0a-9a60-c3ca8f8a9154", | 290 | "id": "dab1a17e-9bf9-4e0a-9a60-c3ca8f8a9154", | ||
291 | "name": "EDNA", | 291 | "name": "EDNA", | ||
292 | "state": "active", | 292 | "state": "active", | ||
293 | "vocabulary_id": null | 293 | "vocabulary_id": null | ||
294 | }, | 294 | }, | ||
295 | { | 295 | { | ||
296 | "display_name": "ENVIRONMENTAL DNA", | 296 | "display_name": "ENVIRONMENTAL DNA", | ||
297 | "id": "db7d5622-d1fc-4d24-91f7-73842c6743b6", | 297 | "id": "db7d5622-d1fc-4d24-91f7-73842c6743b6", | ||
298 | "name": "ENVIRONMENTAL DNA", | 298 | "name": "ENVIRONMENTAL DNA", | ||
299 | "state": "active", | 299 | "state": "active", | ||
300 | "vocabulary_id": null | 300 | "vocabulary_id": null | ||
301 | }, | 301 | }, | ||
302 | { | 302 | { | ||
303 | "display_name": "FIELD-BASED PROTOCOL", | 303 | "display_name": "FIELD-BASED PROTOCOL", | ||
304 | "id": "3fbda4ad-4504-4b3b-9ea5-d6fea74d8730", | 304 | "id": "3fbda4ad-4504-4b3b-9ea5-d6fea74d8730", | ||
305 | "name": "FIELD-BASED PROTOCOL", | 305 | "name": "FIELD-BASED PROTOCOL", | ||
306 | "state": "active", | 306 | "state": "active", | ||
307 | "vocabulary_id": null | 307 | "vocabulary_id": null | ||
308 | }, | 308 | }, | ||
309 | { | 309 | { | ||
310 | "display_name": "QPCR", | 310 | "display_name": "QPCR", | ||
311 | "id": "07ca9de3-732c-4f4b-9458-6bb5aaa124b2", | 311 | "id": "07ca9de3-732c-4f4b-9458-6bb5aaa124b2", | ||
312 | "name": "QPCR", | 312 | "name": "QPCR", | ||
313 | "state": "active", | 313 | "state": "active", | ||
314 | "vocabulary_id": null | 314 | "vocabulary_id": null | ||
315 | }, | 315 | }, | ||
316 | { | 316 | { | ||
317 | "display_name": "THREATENED SPECIES", | 317 | "display_name": "THREATENED SPECIES", | ||
318 | "id": "3429571a-7a40-4cf4-aa0d-ea6f69879ca7", | 318 | "id": "3429571a-7a40-4cf4-aa0d-ea6f69879ca7", | ||
319 | "name": "THREATENED SPECIES", | 319 | "name": "THREATENED SPECIES", | ||
320 | "state": "active", | 320 | "state": "active", | ||
321 | "vocabulary_id": null | 321 | "vocabulary_id": null | ||
322 | } | 322 | } | ||
323 | ], | 323 | ], | ||
324 | "title": "Field-based eDNA analysis to detect the threatened and | 324 | "title": "Field-based eDNA analysis to detect the threatened and | ||
325 | elusive African manatee", | 325 | elusive African manatee", | ||
326 | "type": "dataset", | 326 | "type": "dataset", | ||
t | 327 | "url": null | t | 327 | "url": null, |
328 | "version": "1.0" | ||||
328 | } | 329 | } |