Environmental Data: Alnus glutinosa (L.) Gaertn. and Orientus ishidae ...

Plots description

Characteristics of the monitored vineyards, including the number of sampled common alders and the number of yellow sticky traps (YST) placed in the canopy.

Features of the phytoplasma isolates

Features of the phytoplasma isolates analyzed in this work and reference strains, together with relative GenBank accession numbers and host.

Leafhopper captures in vineyards.

Leafhoppers captured on yellow sticky traps (YST) placed in vineyards and sampled for molecular analyses. Counts may differ if the quality of the individual specimens was deemed as insufficient for the molecular analysis. Specimens of Scaphoideus titanus caught in Camorino were sampled but not analyzed.

Analyzed and infected specimens.

Number of analyzed and 16SrV-infected specimens collected on common alder trees and on yellow sticky traps (YST) placed inside the vineyard and corresponding infection rate.

Results of molecular analyses on a selection of the sampled symptomatic grapevines in Origlio.

Real-time PCR for 16SrV and 16SrXII phytoplasmas, and nested PCR and RFLP digestion (16SrV phytoplasma only). Cq; cycle quantification value; BDL: below detection limit; FDp: “Flavescence dorée” phytoplasma; BNp: “Bois noir” phytoplasma; +: positive response to real-time PCR test; -: negative response to real-time PCR test.

Orientus ishidae (development stages L1-2; L3-5; and imago) caught with sweeping net on common alders per plot.

Number of sampling common alder trees (NAlders) and data regarding captures of O. ishidae (mean, standard deviation (SD), number of observations (NObservations) and total seasonal captures) are reported.

Results of the molecular analyses conducted on the sampled common alder trees in Origlio, Camorino, and Montalbano.

Real-time PCR (conducted on all samples), and nested PCR and RFLP digestion (Origlio only). Cq; cycle quantification value; +: positive response to real-time PCR test; -: negative response to real-time PCR test.